Controlling the Binding Efficiency of Surface Confined Antibodies through the Design of Mixed Self‐Assembled Monolayers

A plethora of different electronic and optoelectronic devices have been developed lately, for biosensing applications (e.g., label-free, fast, easier to operate) based on a detecting interface accommodating the biorecognition elements, anchored by thiolate self-assembled monolayers (SAMs) gold surface. Here, surface plasmon resonance (SPR) characterization anti-p24 SAMs is performed investigate effect SAM structure antibodies’ packing efficiency sensors’ analytical figures merit. Notably, mixed deposited from solution 10:1 3-mercaptopropionic acid 11-mercaptoundecanoic (11MUA) compared that resulting ad hoc synthesized N-(2-hydroxyethyl)-3-mercaptopropanamide (NMPA)/11MUA. Despite improvement in coverage registered using 11MUA/NMPA SAM, latter produces significant decrease binding against human immunodeficiency virus p24 protein. To provide molecular rationale behind SPR data, density functional theory calculations are also undertaken. comprehensive physical view main competing phenomena affecting events at biofunctionalized represented here.